Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), eFluor 450, eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 561
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 660
• FITC
• Functional Grade
• NovaFluor Red 700
• NovaFluor Red 710
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 645
• Request custom conjugation

Product Specific Information

The A7R34 monoclonal antibody reacts with mouse CD127, the high-affinity alpha subunit of the mouse IL-7 receptor. CD127 is expressed by immature B cells, double-negative (CD4-CD8-), single-positive (CD4+ or CD8+) thymocytes, but not double-positive (CD4+CD8+). In mature T cells, CD127 expression is low.
A7R34 inhibits IL-7 binding to its receptor and is used in studies of IL-7 roles in T/B cell development and activation. It blocks SB/199 antibody binding to mouse CD127.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes (≤1 µg/test).
A test is defined as staining a cell sample in 100 µL volume. Cell count typically ranges from 10⁵–10⁸ cells/test. Optimize antibody titration for best performance.

eFluor® 450 is an alternative to Pacific Blue®, emitting at 445 nm when excited at 405 nm (Violet laser). Ensure instrument compatibility.

• Excitation: 405 nm
• Emission: 445 nm
• Laser: Violet
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD127 (Interleukin-7 receptor alpha) is a glycoprotein regulating lymphopoiesis. The IL7R complex consists of alpha and gamma chains; the gamma chain activates signal transduction, while the alpha chain mediates specific signaling.
CD127 promotes proliferation of precursor B cells, thymocytes, and mature T cells. Dysfunction is associated with severe combined immunodeficiency (SCID) variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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