Thermo Fisher Scientific ViraPower HiPerform T-REx Gateway Vector Kit

Invitrogen™ ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit

The ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ tetracycline-regulated lentiviral expression vector, pLenti6.3/TO/V5-DEST™, for easy recombination-based cloning and lentiviral-based, regulated, high-level expression of a target gene in dividing and non-dividing mammalian cells.
The vector includes two key genetic elements — Woodchuck Posttranscriptional Regulatory Element (WPRE) and central Polypurine Tract (cPPT) from the HIV-1 integrase gene — to achieve at least a 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages

• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway™ technology
• Stable, long-term, tetracycline-regulated expression
• Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems

Key Features

• WPRE from woodchuck hepatitis virus increases transgene expression
• cPPT from HIV-1 integrase gene increases viral integration copy number and viral titer
• Combined WPRE and cPPT yield at least four-fold higher protein expression than standard vectors
• Hybrid promoter (CMV + TetO2 sites) for high-level, regulated expression
• Blasticidin selection marker under SV40 promoter for stable selection

Kit Includes

• pLenti6.3/TO/V5-DEST™ vector
• pLenti3.3/TR vector
• pLenti6.3/TO/V5-GW/lacZ plasmid
• Tetracycline
• One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)
• pENTR™ Gus positive control plasmid

Specifications