Thermo Fisher Scientific ViraPower HiPerform Promoterless Gateway Vector Kit

Invitrogen™ ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit

The ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit contains the MultiSite Gateway™-adapted ViraPower™ HiPerform™ promoterless lentiviral expression vector, pLenti6.4/R4R2/V5-DEST™, for easy recombination-based cloning and lentiviral-based high-level expression of a target gene from any promoter of choice in dividing and non-dividing mammalian cells.

The pLenti6.4/R4R2/V5-DEST™ vector includes two key genetic elements, making it a HiPerform™ vector:

• WPRE (Woodchuck Posttranscriptional Regulatory Element)
• cPPT (central Polypurine Tract) from the HIV-1 integrase gene

These elements together produce at least a 4-fold increase in protein expression compared to vectors lacking them.

Advantages

• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Enables easy, simultaneous, recombination-based cloning of multiple DNA fragments in a defined order and orientation using MultiSite Gateway™ technology
• Allows expression of the target gene under the control of a promoter of choice
• Provides stable, long-term expression
• Enhances protein expression up to 4-fold or greater compared to traditional lentiviral expression systems

Key Features

• WPRE increases transgene expression
• cPPT increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer
• Promoterless vector design enables expression under any chosen promoter
• Blasticidin selection marker under PGK promoter for long-term stable expression

Kit Includes

• pLenti6.4/R4R2/V5-DEST™ Vector Kit
• pENTR™ 5’ TOPO™ TA Cloning Kit (Cat. No. K59120)
• One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)
• pENTR™ Gus positive control plasmid

Specifications

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