Thermo Fisher Scientific ViraPower HiPerform T-REx Gateway Expression System

Invitrogen™ ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System

The ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System provides all essential components to generate lentivirus, including vector kit, 293FT cell line, and the support kit.
This system integrates Invitrogen’s ViraPower™ HiPerform™ Lentiviral, T-REx™, and Gateway™ technologies for recombination-based cloning and tetracycline-inducible, high-level expression of target genes in dividing and non-dividing mammalian cells.

The pLenti6.3/TO/V5-DEST vector includes two key genetic elements — WPRE (Woodchuck Posttranscriptional Regulatory Element) and cPPT (central Polypurine Tract) — resulting in at least a 4-fold increase in protein expression compared to vectors lacking these sequences.

Advantages

• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway™ technology
• Stable, long-term, tetracycline-regulated expression
• Enhanced protein expression up to 4-fold or greater compared to traditional lentiviral systems

Key Features

• WPRE and cPPT elements enhance viral titer and transgene expression
• Hybrid CMV/TetO2 promoter enables high-level, regulated expression
• Blasticidin selection marker under SV40 promoter for stable selection

Kit Includes

• ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit (Cat. No. A11144)
• ViraPower™ Bsd Lentiviral Support Kit (K497000)
• 293FT Cell Line (R70007)
• Gateway™ LR Clonase™ II Plus Enzyme Mix (12538120)
• One Shot™ Stbl3™ Chemically Competent E. coli (C737303)
• Geneticin™ (10131035)
• pENTR™ Gus positive control plasmid

Specifications