Invitrogen™

ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System

The ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell자세히 알아보기

The ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen`s ViraPower™ HiPerform™ Lentiviral, T-REx™ and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based, regulated (Tetracycline-inducible), high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3/ TO/V5-DEST vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages

• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway™ technology
• Stable, long-term, tetracycline-regulated expression
• Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems

Key Features

• WPRE (Woodchuck Posttranscriptional Regulatory Element) from the woodchuck hepatitis virus, increases transgene expression and cPPT (central Polypurine Tract) from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
• Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene
• Blasticidin selection marker for stable selection under control of SV40 promoter

Kit includes

• ViraPower™ HiPerform™ T-Rex™ Gateway™ Vector Kit (Cat. No. A11144)
• ViraPower™ Bsd Lentiviral Support Kit (K497000)
• 293FT Cell Line (R70007)
• Gateway™ LR Clonase™ II Plus Enzyme Mix (12538120)
• One Shot™ Stbl3™ Chemically Competent E. coli (C737303)
• Geneticin™ (10131035)
• pENTR™ Gus positive control plasmid

사양

항생제 내성 박테리아Ampicillin (AmpR), Blasticidin (BsdR)

클로닝 방법Gateway

구성 또는 유도성 시스템Inducible

배달 유형Lentiviral

셀 유형293-FT

형식Kit

프로모터CMV/TO

VectorpLenti

Gateway 효소LR Clonase™ II Plus, LR Clonase™ II

용도(장비)Gateway™, HiPerform™, T-REx™, ViraPower™

용도(애플리케이션)Protein Expression

유도제Doxycycline, Tetracycline

주요 기능Viral Expression

반응 수20 Reactions

PCR 효소Proof-Reading Polymerase

제품라인Gateway™, HiPerform™, T-REx™, ViraPower™

제품 유형Mammalian Expression system

단백질 태그V5 Epitope Tag

수량20 Reactions

선택 제제(진핵)Blasticidin

복제 개시점pUC Origin

선택 마커 진핵BsdR

선택 마커 프로모터PGK Promoter

특이 요소WPRE, cPPT

Unit SizeEach