Thermo Fisher Scientific OCT4 Polyclonal Antibody
Thermo Fisher Scientific OCT4 Polyclonal Antibody는 인간 OCT4 단백질을 인식하는 Rabbit IgG 항체로, WB, ICC/IF, Flow, IP, ChIP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 줄기세포 연구 및 iPS 세포 생성 관련 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 159–360 of Human OCT3/4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.62 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544670 |
Product Specific Information
- Recommended positive controls: human ESC
- Predicted reactivity: Mouse (83%), Rat (84%), Cat (96%), Pig (95%), Rabbit (92%), Rhesus Monkey (99%), Chimpanzee (100%), Bovine (95%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
POU5F1, commonly known as Oct-4, is a maternally expressed octamer-binding protein involved in early embryonic development. It plays a key role in maintaining pluripotency and self-renewal in stem cells. Expression of POU5F1, along with Sox2, Klf4, Lin28, and c-Myc, enables the creation of induced pluripotent stem (iPS) cells from somatic cells. Recent studies show that iPS cells can be generated using non-viral expression plasmids, improving safety for regenerative medicine applications.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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