Thermo Fisher Scientific IgM (Immunoglobulin Mu Heavy Chain) Recombinant Mouse Monoclonal Antibody (rIGHM, 1623)
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Recombinant mouse monoclonal antibody recognizing human IgM (mu heavy chain). Validated for IHC, ICC/IF, and Flow Cytometry. Produced in HEK293 cells, purified via Protein A/G, and supplied as a liquid form for research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rIGHM, 1623 |
| Immunogen | Recombinant human full-length IGHM protein (UniProt ID: P01871-2; antigen range: 1–474) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For IHC (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
IgM is expressed intracellularly during early stages of B lymphopoiesis and on the surface of more mature B cells in the bone marrow and peripheral B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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