Thermo Fisher Scientific IgM (Immunoglobulin Mu Heavy Chain) Recombinant Mouse Monoclonal Antibody (rIGHM, 1623)
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인간 IgM 단백질을 인식하는 재조합 마우스 단일클론 항체입니다. IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합합니다. HEK293 세포에서 발현되었으며, Protein A/G로 정제된 고순도 항체입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rIGHM, 1623 |
| Immunogen | Recombinant human full-length IGHM protein (UniProt ID: P01871-2, 1–474 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody is tested in direct ELISA.
- For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
IgM is expressed intracellularly during early stages of B lymphopoiesis and then on the surface of more mature B cells in the bone marrow and peripheral B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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