Thermo Fisher Scientific IgM (Immunoglobulin Mu Heavy Chain) Recombinant Mouse Monoclonal Antibody (rIM260)
Recombinant mouse monoclonal antibody (clone rIM260) specific for human IgM. Validated for IHC (paraffin) at 1–2 µg/mL. Produced in HEK293 cells and purified by Protein A/G. Supplied as a liquid at 200 µg/mL in PBS with BSA and sodium azide. For resear...
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rIM260 |
| Immunogen | Recombinant full-length human IGHM protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
IgM is expressed intracellularly during early stages of B lymphopoiesis and then appears on the surface of more mature B cells in the bone marrow and peripheral B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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