Thermo Fisher Scientific DEFA1 Polyclonal Antibody, PeproTech
인간 DEFA1 단백질을 인식하는 염소 유래 폴리클로날 항체로, Western blot과 ELISA에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 무보존제 PBS 용액으로 제공. 연구용으로만 사용.
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Thermo Fisher Scientific DEFA1 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human NP-1 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929360 |
Product Specific Information
- AA Sequence of recombinant protein: ACYCRIPACI AGERRYGTCI YQGRLWAFCC
- Preparation: Produced from sera of goats immunized with highly pure Recombinant Human NP-1. Anti-Human NP-1-specific antibody was purified by affinity chromatography employing an immobilized Human NP-1 matrix.
- Sandwich ELISA:
To detect Human NP-1 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required.
This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NP-1 (500-P126GBt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human NP-1. - Western Blot:
To detect Human NP-1 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used with compatible secondary reagents, the detection limit for Recombinant Human NP-1 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Defensins are a family of microbicidal and cytotoxic peptides involved in host defense. They are abundant in neutrophil granules and found in mucosal epithelia such as the intestine, respiratory tract, urinary tract, and vagina.
Members of the defensin family share high sequence similarity and a conserved cysteine motif.
The gene encoding defensin alpha 1 is located on chromosome 8 and differs from defensin alpha 3 by only one amino acid. Both genes are subject to copy number variation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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