Thermo Fisher Scientific PAK2 Monoclonal Antibody (3B5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human, Non-human primate

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3B5

Immunogen

Purified recombinant fragment of PAK2 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10987125

Product Specific Information

MA5-15527 targets PAK2 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human and Non-human primate samples.

The MA5-15527 immunogen is purified recombinant fragment of PAK2 expressed in E. Coli.

MA5-15527 detects PAK2 which has a predicted molecular weight of approximately 61kDa.

Target Information

PAK2 (serine/threonine-protein kinase PAK2) is a serine/threonine kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis, or proliferation. It acts as a downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformation change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.