Thermo Fisher Scientific Ki-67 Monoclonal Antibody (4A1)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA5-15525 targets Ki-67 in indirect ELISA and IHC applications, showing reactivity with human samples.
The immunogen is a synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human Ki-67, conjugated to KLH.
MA5-15525 detects Ki-67, which has a predicted molecular weight of approximately 358 kDa.

Target Information

Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly in late G1, S, G2, and M phases.
It associates with euchromatin at the perichromosomal layer, centromeric heterochromatin, and the nucleolus via its forkhead-associated (FHA) domain.
Ki-67 shows cell cycle–dependent localization: perinucleolar in G1, nuclear matrix in G2, and chromosomal during M phase.
It serves as a proliferation marker since it is absent in G0 cells but increases from G1 through mitosis.
Ki-67 antibodies are widely used to assess proliferative activity in neoplasms, correlating with tumor grade and prognosis.
Commonly used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.