Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), PerCP-Cyanine5.5, eBioscience
PerCP-Cyanine5.5로 결합된 Rat IgG2a kappa 동형 대조 항체로, 유세포 분석용 음성 대조에 적합합니다. 488 nm에서 여기되고 695 nm에서 방출됩니다. 0.2 mg/mL 농도의 액상 제품이며, 4°C 어두운 곳에 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBR2a |
| Conjugate | PerCP-Cyanine5.5 |
| Excitation / Emission Max | 489 / 679 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_906259 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506, 570, 615, 660
- FITC
- Functional Grade
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
Description:
The monoclonal rat IgG2a kappa is useful as an isotype control immunoglobulin.
Applications Reported:
PerCP-Cy5.5 Rat IgG2a Isotype Control has been reported for use in flow cytometric analysis.
Applications Tested:
This isotype control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.
Fluorescence Characteristics:
- Excitation: 488 nm
- Emission: 695 nm
- Laser: Blue Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Rat IgG2a is an immunoglobulin subclass produced by the rat immune system, known for complement activation and antibody-dependent cell-mediated cytotoxicity (ADCC).
It has a molecular weight of approximately 160 kDa and consists of two heavy and two light chains.
Commonly used as a control or reference antibody in experiments involving rat models, including flow cytometry and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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