Thermo Fisher Scientific SLC5A5 Monoclonal Antibody (FP5), PerCP
SLC5A5 단백질(hNIS)을 인식하는 FP5 클론의 마우스 단클론항체로 PerCP 형광 표지형입니다. WB, IHC, ICC 등 다양한 응용에 사용 가능하며, 인간·마우스·랫트 반응성을 보입니다. 단백질 G 정제, 1 mg/mL 농도, 4°C 보관.
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Thermo Fisher Scientific SLC5A5 Monoclonal Antibody (FP5), PerCP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FP5 |
| Immunogen | Mannose binding protein hNIS fusion (AA468–643) |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931948 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45494 was sufficient for detection of hNIS in 20 µg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 97 kDa, non-glycosylated version at 68 kDa. Other minor bands associated with hNIS at 160 kDa, and degradation products at approximately 30 kDa and 15 kDa.
Target Information
Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane-bound glycoprotein with 13 membrane-spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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