Thermo Fisher Scientific CXCL3 Polyclonal Antibody, PeproTech

Applications

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-gamma (CXCL3). Anti-Human GRO-gamma (CXCL3)-specific antibody was purified by affinity chromatography employing an immobilized Human GRO-gamma (CXCL3) matrix.

Sandwich ELISA:
To detect Human GRO-gamma (CXCL3) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human GRO-gamma (CXCL3) (500-P105Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human GRO-gamma (CXCL3).

Western Blot:
To detect Human GRO-gamma (CXCL3) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human GRO-gamma (CXCL3) is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

CXCL3 may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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