Thermo Fisher Scientific A-Raf Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

• It is not recommended to aliquot this antibody.
• This antibody is not cross-reactive with c-Raf or B-Raf.

Target Information

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity and is broadly expressed in nearly all cell lines tested to date. It is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus.
The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain.
A-Raf, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at the lowest level in brain tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.