Thermo Fisher Scientific Phospho-CREB/ATF1 (Ser133, Ser63) Monoclonal Antibody (10E9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

-

ELISA (ELISA)

0.05 µg/mL

-

ChIP assay (ChIP)

5 µL/10^6 cells

-

Product Specifications

Species Reactivity

Dog, Human, Mouse

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

10E9

Immunogen

A synthetic phosphopeptide corresponding to amino acids 125-135 surrounding the Ser133 phosphorylation site of human CREB if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CREB/ATF1,uniProtId:P16220-2,ncbiNodeId:9606,antigenRange:125-135,antigenLength:327,antigenImageFileName:MA1-114_CREBATF1_P16220-2_House_mouse.svg,antigenImageFileNamePDP:MA1-114_CREBATF1_P16220-2_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1 mg/mL

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.2, with sucrose, PEG, 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2536825

Product Specific Information

This antibody recognizes CREB protein phosphorylated at serine 133 and ATF1 phosphorylated at serine 63. The antibody was purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.

Reconstitute with 1 mL H2O (15 min, RT)

Aliquot and freeze in liquid nitrogen. Antibody can be stored frozen at -80ºC for up to 1 year. Thaw aliquots at 37ºC; thawed aliquots may be stored at 4ºC for up to 3 months. Avoid repeated freeze/thaw cycles.

This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Target Information

Cyclic AMP response element binding protein (CREB) is a basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44/42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.