Thermo Fisher Scientific HLA-A2 Monoclonal Antibody (BB7.2), APC, eBioscience
인간 HLA-A2를 인식하는 BB7.2 단클론 항체로, APC 형광표지되어 유세포 분석(Flow Cytometry)에 최적화됨. 블로킹 활성 보고됨. 5 µL/test로 사용 가능하며, 4°C 암소 보관. 연구용으로만 사용.
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Thermo Fisher Scientific HLA-A2 Monoclonal Antibody (BB7.2), APC, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.5 µg)/test
Publications: View 8 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BB7.2 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151522 |
Additional Formats
- APC-eFluor 780
- NovaFluor Blue 510
- NovaFluor Blue 585
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Yellow 570
- NovaFluor Yellow 660
- NovaFluor Yellow 730
- NovaFluor Yellow 755
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
This BB7.2 monoclonal antibody recognizes the human major histocompatibility complex (MHC) class I molecule HLA-A2, a member of the HLA-A family.
MHC class I consists of a single alpha chain with three domains and associates with beta 2-microglobulin, expressed on all nucleated cells.
- Exhibits blocking activity and also recognizes HLA-A28.
- Reported for use in flow cytometric analysis.
- Pre-titrated and tested on normal human peripheral blood cells.
- Recommended usage: 5 µL (0.5 µg) per test (100 µL final volume).
- Cell number range: 10⁵–10⁸ cells/test.
- Excitation: 633–647 nm; Emission: 660 nm; Laser: Red.
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
HLA-A is part of the HLA class I heavy chain family, forming a heterodimer with beta-2 microglobulin.
It plays a key role in the immune system by presenting peptides from the endoplasmic reticulum lumen and is expressed in nearly all cells.
- Heavy chain: ~45 kDa, composed of 8 exons.
- Exons 2 and 3 determine peptide-binding specificity.
- Polymorphism typing is essential for bone marrow and kidney transplantation.
- Hundreds of HLA-A alleles have been described.
- HLA/MHC antibodies are crucial in Immunopeptidomics, aiding neoantigen identification via LC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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