Thermo Fisher Scientific Cyclin H Polyclonal Antibody
Cyclin H 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, ELISA, IP에 사용 가능. Human 시료 반응성. Affinity Chromatography로 정제된 Liquid 형태로, PBS/glycerol buffer에 보관. 세포주 Jurkat, K-562 등에서 검증됨.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | |
| ELISA | 1 µg/mL | |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–323 of human Cyclin H (NP_0012301) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.12 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS (pH 7.3) with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804247 |
Product Specific Information
Immunogen sequence:
MYHNSSQKRH WTFSSEEQLA RLRADANRKF RCKAVANGKV LPNDPVFLEP HEEMTLCKYY EKRLLEFCSV FKPAMPRSVV GTACMYFKRF YLNNSVMEYH PRIIMLTCAF LACKVDEFNV SSPQFVGNLR ESPLGQEKAL EQILEYELLL IQQLNFHLIV HNPYRPFEGF LIDLKTRYPI LENPEILRKT ADDFLNRIAL TDAYLLYTPS QIALTAILSS ASRAGITMES YLSESLMLKE NRTCLSQLLD IMKSMRNLVK KYEPPRSEEV AVLKQKLERC HSAELALNVI TKKRKGYEDD DYVSKKSKHE EEEWTDDDLV ESL
Positive Samples: Jurkat, K-562, SW620, HT-29, MCF7
Cellular Location: Nucleus
Target Information
The protein encoded by this gene belongs to the highly conserved cyclin family, characterized by periodic changes in protein abundance through the cell cycle. Cyclins regulate CDK kinases, and different cyclins have distinct expression and degradation patterns that coordinate mitotic events. Cyclin H forms a complex with CDK7 kinase and ring finger protein MAT1, functioning as a CDK-activating kinase (CAK). This complex is part of TFIIH and RNA polymerase II complexes, linking transcriptional regulation and cell cycle control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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