Thermo Fisher Scientific Synaptotagmin 1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 84-157 of human SYT1 (NP_0056301) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Synaptotagmin 1,uniProtId:P21579-1,ncbiNodeId:9606,antigenRange:84-157,antigenLength:422,antigenImageFileName:PA5-87551_Synaptotagmin_1_P21579-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87551_Synaptotagmin_1_P21579-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.46 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804245

Product Specific Information

Immunogen sequence: LFKKKNKKKG KEKGGKNAIN MKDVKDLGKT MKDQALKDDD AETGLTDGEE KEEPKEEEKL GKLQYSLDYD FQNN

Positive Samples: SW620, A673; Cellular Location: Cytoplasm, Cytoplasmic vesicle, Single-pass membrane protein, chromaffin granule membrane, secretory vesicle, synaptic vesicle membrane

Target Information

Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Studies indicate that synaptotagmin`s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane. It has been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Synaptotagmin 1 may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. Further, Synaptotagmin 1 binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has been reported. Synaptotagmin 1 can also bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Diseases associated with the dysfunction of Synaptotagmin 1 include food-borne botulism and infant botulism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.