Thermo Fisher Scientific Kappa Light Chain/IGKC Recombinant Mouse Monoclonal Antibody (rKLC709)
인간 Kappa Light Chain을 인식하는 재조합 마우스 단클론 항체. IHC(P) 및 Peptide Array에 사용 가능. HEK293 세포에서 발현된 IgG1, kappa 형식의 항체로, Protein A/G로 정제됨. PBS(pH 7.4) 용액 형태로 제공되며 방부제 무첨가.
- 카탈로그번호
- 3514-MSM10-P1ABX
- 판매단위
- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rKLC709 |
| Immunogen | Recombinant full-length human Ig kappa chain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Immunoglobulin classes share a basic structure of four polypeptide chains: two heavy chains and two light chains (kappa or lambda, each ~22.5 kDa).
Kappa and lambda chains consist of variable and constant regions, distinguishable by the antigenic properties of their constant regions.
The kappa to lambda ratio is approximately 70:30.
Antibodies to the kappa light chain are useful for identifying leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas.
Demonstration of clonality in lymphoid infiltrates indicates malignancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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