Thermo Fisher Scientific Kappa Light Chain Recombinant Mouse Monoclonal Antibody (rKLC264)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five types) and two light chains (kappa, lambda; both having a molecular weight of 22.5 kDa).
Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
The ratio of kappa to lambda is 70:30.
Antibodies to the kappa light chain are reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas.
Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.