Thermo Fisher Scientific VAMP2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

View 3 publications 3 publications

Immunohistochemistry (IHC)

1:1000

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Published species

Dog

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from aa region 10-60 of human VAMP2 conjugated to blue carrier protein was used as the antigen. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:VAMP2,uniProtId:P63027-1,ncbiNodeId:9606,antigenRange:10-60,antigenLength:116,antigenImageFileName:OSS00035W_VAMP2_P63027-1_Rabbit.svg,antigenImageFileNamePDP:OSS00035W_VAMP2_P63027-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1960853

Product Specific Information

Glycerol (1:1) may be added for additional stability.

Reconstitute in 100 µL of sterile water.

The VAMP2 peptide shares 93% shared identity with VAMP1. The peptide is homologous in many other species including rat, mouse and xenopus.

Target Information

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer`s disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP-2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.