Thermo Fisher Scientific PLK1 Polyclonal Antibody
PLK1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, ICC/IF, IP 등 다양한 응용에 적합합니다. 세포 분열 및 암 연구에 유용하며, 1 mg/mL 액상 형태로 제공됩니다. 4°C 보관, 습식 얼음 배송 조건입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–4 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 325 and 375 of human Polo-like kinase (PLK1, UniProt ID: P53350-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note:
- For ICC, formaldehyde fixation is recommended.
- Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Target Information
PLK1 (Polo-like kinase 1) is a serine/threonine kinase involved in cell mitosis and serves as a marker of cell proliferation. It plays a critical role in carcinogenesis. PLK1 acts by binding and phosphorylating proteins that are already phosphorylated on a specific motif recognized by the POLO box domains.
PLK1 phosphorylates multiple substrates including BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, TP73, TPT1, and WEE1.
It plays a key role in centrosome function and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1, and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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