Thermo Fisher Scientific EBI3 Monoclonal Antibody (V1.4H6.29)

Applications and Tested Dilution

Product Specifications

Additional Formats

• Biotin
• Request custom conjugation

Product Specific Information

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for mouse EBI3 protein. A BLAST analysis was used to suggest cross-reactivity with EBI3 from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with EBI-3 from other sources has not been determined.

Target Information

EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, mediated through the control of iNKT cell function.
EBI3 dimerizes with p28 and p35 subunits of IL-12 to form IL-27 and IL-35, respectively. IL-27, produced by activated antigen-presenting cells upon TLR ligation, negatively regulates Th17 cell differentiation.
EBI3 is widely expressed and transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses. It inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction.
EBI3 may play a role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with reduced Th2 cytokine production, which regulates allergic airway inflammation in asthma.
Targeted deletion of EBI3 protects mice from lung metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.