Thermo Fisher Scientific HIF1A Polyclonal Antibody

Applications

• Western Blot (WB): Tested at 1 µg/mL

Product Specifications

Product Specific Information

This antibody is predicted to react with mouse, rat, bovine, and porcine based on 100% sequence homology; 84% sequence homology with xenopus.

Target Information

HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under reduced oxygen tension. HIF-1 is a heterodimer consisting of alpha and beta subunits, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors.
HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor (VEGF), and other genes that enhance oxygen delivery or facilitate metabolic adaptation.
HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. Hypoxia induces p53 accumulation, which interacts with HIF1-alpha and reduces its expression by promoting MDM2-mediated ubiquitination and proteasomal degradation.
Recent studies suggest that HIF1-alpha–induced NOX4 helps maintain ROS levels after hypoxia and promotes hypoxia-induced proliferation. In humans, HIF1A is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300, and p300/CBP-HIF complexes induce hypoxia-responsive genes, including VEGF.
Hypoxia contributes significantly to diseases such as myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and chronic obstructive pulmonary disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.