Thermo Fisher Scientific Phospho-EIF2S1 (Ser52) Monoclonal Antibody (S.674.5)
Phospho-EIF2S1 (Ser52) 단일클론 항체로 인간, 생쥐 등 다양한 종에 반응. Western blot, IHC, IP 등 다양한 응용 가능. 고순도 친화 크로마토그래피 정제. 액상 형태로 -20°C 보관. 연구용으로 스트레스 반응 관련 단백질 인산화 연구에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 9 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:75–1:300 | View publication |
| Immunoprecipitation (IP) | 1:50 | View publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Fruit fly, Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S.674.5 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pSer52 of human eIF2α |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 97 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10983400 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
EIF2α (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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