Thermo Fisher Scientific Robo3 Polyclonal Antibody, FITC
FITC로 결합된 Robo3 폴리클로날 항체로, Western blot, ELISA, IP 등 다양한 분석에 적합합니다. 사람, 소, 영장류, 판다 반응성이 있으며, 고순도의 친화 크로마토그래피 정제 제품입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Non-human primate, Panda |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to unique amino acid sequence on human RIG1 protein |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The ROBO3 gene is part of the Roundabout (ROBO) family, which regulates neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins belong to the immunoglobulin transmembrane receptor superfamily. Their ligands, SLIT proteins (1–3), are secreted chemorepellants that interact with ROBO proteins to regulate processes such as myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, vasculogenesis, and neurogenesis.
ROBO3 features an extracellular domain with five Ig-like loops and three fibronectin type III motifs, a transmembrane segment, and a cytoplasmic tail with conserved signaling motifs CC0, CC2, and CC3 (but lacking CC1). ROBO3 controls axonal navigation at the ventral midline of the neural tube. In mice, loss of Robo3 leads to failure of commissural axons to cross the midline. Mutations in ROBO3 cause horizontal gaze palsy with progressive scoliosis (HGPPS), an autosomal recessive disorder characterized by congenital absence of horizontal gaze and progressive spinal curvature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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