Thermo Fisher Scientific Robo3 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Target Information

The ROBO3 gene belongs to the Roundabout (ROBO) family, which regulates neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are part of the immunoglobulin transmembrane receptor superfamily. SLIT proteins (1–3) act as ligands for ROBO proteins, and their interactions influence myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, vasculogenesis, and neurogenesis.

ROBO3 features an extracellular domain with five immunoglobulin-like loops and three fibronectin type III motifs, a transmembrane region, and a cytoplasmic tail with conserved signaling motifs (CC0, CC2, CC3). Unlike other ROBO family members, ROBO3 lacks the CC1 motif. It plays a key role in axonal navigation at the ventral midline of the neural tube. In mice, loss of Robo3 leads to failure of commissural axons to cross the midline in the spinal cord and hindbrain. Mutations in ROBO3 are associated with horizontal gaze palsy with progressive scoliosis (HGPPS), an autosomal recessive disorder characterized by absence of horizontal gaze, progressive scoliosis, and failure of corticospinal and somatosensory tracts to cross the medulla midline.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.