Thermo Fisher Scientific Calreticulin Recombinant Rabbit Monoclonal Antibody (10J1K8)
Calreticulin 단백질을 인식하는 토끼 유래 재조합 단클론 항체. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 고순도 Affinity chromatography 정제. -20°C 보관, 동결/해동 반복 피할 것.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 10J1K8 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 18–203 of human Calreticulin (NP_004334.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3094165 |
Product Specific Information
Immunogen sequence:
EPAVYFKEQF LDGDGWTSRW IESKHKSDFG KFVLSSGKFY GDEEKDKGLQ TSQDARFYAL SASFEPFSNK GQTLVVQFTV KHEQNIDCGG GYVKLFPNSL DQTDMHGDSE YNIMFGPDIC GPGTKKVHVI FNYKGKNVLI NKDIRCKDDE FTHLYTLIVR PDNTYEVKID NSQVESGSLE DDWDFL
Target Information
Calreticulin (Calr) is a calcium-binding chaperone that promotes folding, oligomeric assembly, and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all monoglucosylated glycoproteins synthesized in the ER. It also interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Additionally, Calr may be involved in maternal gene expression regulation and oocyte maturation through calcium homeostasis regulation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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