Thermo Fisher Scientific Zap-70 Monoclonal Antibody (ZAP-03)
ZAP-70 단백질 티로신 키나아제 검출용 mouse monoclonal antibody. Western blot, ICC/IF, Flow cytometry에 사용 가능. 인간 및 마우스 반응성. Protein A 정제, 1 mg/mL 농도, PBS buffer에 보관. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 2–5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ZAP-03 |
| Immunogen | Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70 with histidine tag |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1088337 |
Product Specific Information
This antibody reacts with ZAP70, a 70 kDa protein tyrosine kinase expressed in T and NK cells (intracellular antigen). ZAP70 is susceptible to degradation; therefore, freshly prepared cell lysates with protease inhibitors are recommended to avoid non-specific staining from degradation products.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family, expressed exclusively in T-cells and natural killer cells. It is essential for T-cell receptor activation. Upon TCR engagement, ZAP70 is phosphorylated at tyrosines 292, 315, and 319 within the interdomain B region. Phosphorylation at tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70 function in lymphocyte signaling, while phosphorylation at tyrosine 292 attenuates signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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