Thermo Fisher Scientific STIM1 Monoclonal Antibody (CDN3H4)
STIM1 단백질을 인식하는 Mouse monoclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합하며, Protein A로 정제된 액상 형태로 제공됩니다. 4°C 보관, 동결 금지.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 8 publications |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | View 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CDN3H4 |
| Immunogen | Synthetic peptide corresponding to the C-terminal cytoplasmic part of STIM1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2197884 |
Product Specific Information
This antibody reacts with a cytoplasmic epitope of human and rodent STIM1, an 84 kDa essential and conserved regulator of store-operated Ca²⁺ channel function.
- Western Blot: Suitable under both reducing and non-reducing conditions
- Immunocytochemistry: Compatible with methanol-acetone fixation
Target Information
STIM1 (Stromal Interacting Molecule 1) acts as a calcium sensor within the endoplasmic reticulum (ER). Upon calcium depletion, STIM1 oligomerizes and interacts with Orai1 at the plasma membrane to mediate calcium influx into the cytoplasm.
STIM1 is associated with microtubule growth ends and contributes to ER tubule extension.
Although its interaction mechanism with Orai1 is not fully elucidated, STIM1 is recognized as a key regulator of store-operated calcium entry (SOCE).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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