Thermo Fisher Scientific AFP Recombinant Rabbit Monoclonal Antibody (2B8Z2)
Recombinant rabbit monoclonal antibody against human AFP for WB and ELISA applications. High specificity and sensitivity. Supplied as liquid, unconjugated form. Suitable for research use only. Stable at -20°C with 50% glycerol buffer.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2B8Z2 |
| Immunogen | Recombinant fusion protein corresponding to amino acids 19–609 of human Alpha-Fetoprotein (AFP) (NP_001125.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.53 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA and 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3093078 |
Product Specific Information
Immunogen sequence:
RTLHRNEYGI ASILDSYQCT AEISLADLAT IFFAQFVQEA TYKEVSKMVK DALTAIEKPT GDEQSSGCLE NQLPAFLEEL CHEKEILEKY GHSDCCSQSE EGRHNCFLAH KKPTPASIPL FQVPEPVTSC EAYEEDRETF MNKFIYEIAR RHPFLYAPTI LLWAARYDKI IPSCCKAENA VECFQTKAAT VTKELRESSL LNQHACAVMK NFGTRTFQAI TVTKLSQKFT KVNFTEIQKL VLDVAHVHEH CCRGDVLDCL QDGEKIMSYI CSQQDTLSNK ITECCKLTTL ERGQCIIHAE NDEKPEGLSP NLNRFLGDRD FNQFSSGEKN IFLASFVHEY SRRHPQLAVS VILRVAKGYQ ELLEKCFQTE NPLECQDKGE EELQKYIQES QALAKRSCGL FQKLGEYYLQ NAFLVAYTKK APQLTSSELM AITRKMAATA ATCCQLSEDK LLACGEGAAD IIIGHLCIRH EMTPVNPGVG QCCTSSYANR RPCFSSLVVD ETYVPPAFSD DKFIFHKDLC QAQGVALQTM KQEFLINLVK QKPQITEEQL EAVIADFSGL LEKCCQGQEQ EVCFAEEGQK LISKTRAALG V
Target Information
Alpha-Fetoprotein (AFP) is a major plasma protein produced by the yolk sac, intestinal tract, and liver during fetal development. In adults, AFP expression is often associated with hepatoma or teratoma, though hereditary persistence can occur without pathology. AFP functions as the fetal counterpart of serum albumin, with both genes located tandemly on chromosome 4. AFP exists in monomeric, dimeric, and trimeric forms, and can bind copper, nickel, fatty acids, and bilirubin. Elevated AFP levels are used as biomarkers in screening for neural tube defects and are associated with hepatocellular carcinoma, teratoblastoma, colorectal, pancreatic cancers, and germ cell tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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