Thermo Fisher Scientific CD14 Monoclonal Antibody (MEM-15), NovaFluor Blue 660-40S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (1.0 µg)/test

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Product Specifications

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Product Specific Information

Description:
The MEM-15 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, interfollicular macrophages, and some dendritic cells. Complexes of LPS and LBP bind with high affinity to monocytes through surface CD14.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (1.0 µg) per test (100 µL final volume). Suitable for 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining with NovaFluor conjugates.
• Add 5 µL per sample to antibody cocktails/master mixes before combining with cells.
• For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology based on novel nucleic acid dye structures, engineered for optimal fluorescent signatures with minimized spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

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Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein constitutively expressed on mature monocytes, macrophages, and neutrophils. It functions as a multifunctional lipopolysaccharide receptor and exists in both membrane-bound and soluble forms. CD14 binds LPS via LBP and participates in immune responses, including recognition of apoptotic cells and modulation of allergic, inflammatory, and infectious processes.
Alternative splicing results in multiple transcript variants encoding the same isoform.
Diseases associated with CD14 dysfunction include Mycobacterium chelonae infection and croup.

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For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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Additional NovaFluor Formats

• NovaFluor Blue 510
• NovaFluor Blue 530
• NovaFluor Blue 585
• NovaFluor Blue 610-30S
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Yellow 570
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 690
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• Custom conjugation available upon request

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