Thermo Fisher Scientific Phospho-RNA Polymerase II (Ser5) Polyclonal Antibody
RNA Polymerase II Ser5 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 사용 가능. 액상 형태로 제공되며 4°C 보관. 인산화된 RNA Pol II 연구에 적합한 고특이성 항체.
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Phospho-RNA Polymerase II (Ser5) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 1–5 µg/mg lysate |
Product Specifications
| Specification | Detail |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phosphorylated synthetic peptide representing a portion of Human RNA Polymerase II around serine 5 of the C-terminal repeat YSPTSPS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Drosophila melanogaster
Target Information
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs.
POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II.
The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS.
Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle.
Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.