Thermo Fisher Scientific RNA Polymerase II Polyclonal Antibody
Thermo Fisher Scientific의 RNA Polymerase II Polyclonal Antibody는 Human 및 Mouse에 반응하는 Rabbit IgG 기반 항체로, WB, IP, ChIP 등 다양한 어플리케이션에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 4°C에서 보관합니다.
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Applications
Western Blot (WB)
- Assay-dependent
Immunoprecipitation (IP)
- 2–10 µg/mg lysate
ChIP assay (ChIP)
- 8 µg/IP
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 754–804 of Human RNA Polymerase II |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs.
POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II.
The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS.
Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle.
Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(원본 이미지: A304-405A_RNA_pol_II_CTD_P24928-1_Rabbit.svg, A304-405A_RNA_pol_II_CTD_P24928-1_Rabbit_PDP.jpeg)
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