Thermo Fisher Scientific UNG Monoclonal Antibody (OTI2B5), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These isoforms are produced by alternative splicing and use of two differentially regulated promoters (PA and PB).

• UNG1 and UNG2 cDNAs are similar in size (2061 and 2058 bp) and cannot be resolved as distinct species in gel electrophoresis.
• UNG2 differs from UNG1 by 44 amino acids at the N-terminus, which are not required for catalytic activity.
• The UNG gene products repair mutagenic U:G mispairs caused by cytosine deamination; for example, UNG2 removes misincorporated dUMP residues.
• Expression levels of UNG1 and UNG2 vary by cell and tissue type, and are higher in proliferating cells.
• UNG1 is a 304 amino acid protein; UNG2 is a 313 amino acid protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.