
Thermo Fisher Scientific ATP6V1F Polyclonal Antibody
Thermo Fisher Scientific의 ATP6V1F Polyclonal Antibody는 인간 V-ATPase F 서브유닛을 인식하는 Rabbit IgG 항체입니다. Western blot에 최적화되어 있으며, 다양한 종에 높은 예측 반응성을 보입니다. 항원 친화 크로마토그래피로 정제된 액상 형태로 제공됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–119 of Human V-ATPase F |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2544992 |
Product Specific Information
- Recommended positive controls: U87-MG
- Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (82%), Xenopus laevis (83%), Chimpanzee (100%), Bovine (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATP6V1F encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme mediating acidification of eukaryotic intracellular organelles.
V-ATPase-dependent organelle acidification is essential for processes such as:
- Protein sorting
- Zymogen activation
- Receptor-mediated endocytosis
- Synaptic vesicle proton gradient generation
V-ATPase consists of:
- V1 domain (cytosolic): three A, three B, two G, plus C, D, E, F, and H subunits — contains ATP catalytic site
- V0 domain (transmembrane): subunits a, c, c′, c″, and d
Additional isoforms of many V1 and V0 subunits are encoded by multiple genes or alternatively spliced transcripts.
ATP6V1F encodes the V1 domain F subunit protein.
⚠ WARNING: This product can expose you to chemicals including mercury, known to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
제품 이미지
(해당 항원 구조 이미지: PA5-27516_ATP6V1F_Q16864-1_Rabbit.svg 및 PA5-27516_ATP6V1F_Q16864-1_Rabbit_PDP.jpeg)
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