Thermo Fisher Scientific FEN1 Polyclonal Antibody
FEN1 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal antibody로, WB, IHC, ICC, IP, PLA 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피 정제. 4°C 단기, -20°C 장기 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
| in situ PLA (PLA) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 169–380 of Human FEN1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544994 |
Product Specific Information
- Recommended positive controls: HepG2, NIH-3T3, JC, BCL-1, Raw264.7, PC-12
- Predicted reactivity: Mouse (96%), Rat (95%), Chicken (81%), Sheep (94%), Rhesus Monkey (98%), Bovine (96%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease cleaving branched DNA structures containing single-stranded 5′ flaps or as an exonuclease on DNA nicks and gaps. Its activities are stimulated by association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 plays essential roles in DNA replication and repair and is required in cell-free DNA replication systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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