Thermo Fisher Scientific PSD-95 Monoclonal Antibody (7E3-1B8)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA1-046 detects post synaptic density 95 (PSD-95) from human, rat, Xenopus laevis, and mouse tissues.
It has been successfully used in Western blot, immunofluorescence, immunocytochemistry, immunohistochemistry, flow cytometry, and immunoprecipitation procedures.
By Western blot, this antibody detects an ~95 kDa protein representing PSD-95 from rat brain extracts.
Immunofluorescence staining of PSD-95 in rat hippocampal cells with MA1-046 yields a staining pattern coincident with NMDA receptor.

For immunofluorescence staining, fixation with cold methanol is recommended.
The immunogen is purified recombinant rat PSD-95.

This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit.
The antibody sold separately may have slightly different performance and may need optimization for best results.

Target Information

Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is a membrane-associated protein found in the postsynaptic density of forebrain neurons and certain presynaptic structures in the cerebellum.
PSD-95 contains three PDZ domains, an SH3 domain, and a guanylate kinase-like (GuK) domain.

It participates in clustering of proteins such as NMDA receptors and Shaker-type potassium channels at the synaptic membrane of CNS neurons.
Two main modes of interaction exist between PSD-95 and other proteins:

• NMDA receptors and Shaker-type potassium channels share a C-terminal T/SXV motif that binds to PDZ domains of PSD-95.
• Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but has its own PDZ domain, associates with PSD-95 through a PDZ-PDZ interaction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.