Thermo Fisher Scientific PSD-95 Monoclonal Antibody (7E3-1B8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 69 publications 69 publications

Immunohistochemistry (IHC)

-

View 66 publications 66 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:500

View 94 publications 94 publications

Flow Cytometry (Flow)

2 µg/test

-

Immunoprecipitation (IP)

Assay-dependent

View 10 publications 10 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Functional Assay (Functional)

-

View 1 publication 1 publication

in situ PLA (PLA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat, Xenopus

Published species

Avian, Bovine, Chicken, Human, Invertebrate, Marsupial, Mouse, Non-human primate, Rabbit, Rat, Zebrafish

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

7E3-1B8

Immunogen

Purified recombinant rat PSD-95.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2092361

Product Specific Information

MA1-046 detects post synaptic density 95 (PSD-95) from human, rat, xenopus laevis and mouse tissues.

MA1-046 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, immunohistochemistry, flow cytometry and immunoprecipitation procedures. By Western blot, this antibody detects an ~95 kDa protein representing PSD-95 from rat brain extracts. Immunofluorescence staining of PSD-95 in rat hippocampal cells with MA1-046 yields a staining pattern coincident with NMDA receptor.

In immunofluorescence staining, fixation with cold methanol is recommended.

The MA1-046 immunogen is purified recombinant rat PSD-95.

This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Target Information

Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.