Thermo Fisher Scientific USP28 Monoclonal Antibody (A1F8)

Applications

• Western Blot (WB): 1:500–1:2,000 dilution

Product Specifications

Product Specific Information

• Positive controls: K562 cell lysates, A549 cell lysates.

Target Information

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity.

An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families:

1. Ubiquitin C-terminal hydrolases
2. Ubiquitin-specific processing proteases (USPs)
3. OTU-domain ubiquitin-aldehyde-binding proteins
4. Jab1/Pad1/MPN-domain-containing metallo-enzymes

Among these, USPs represent the most widespread deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from conjugated proteins and display conserved catalytic domains containing Cys and His boxes, with divergent N- or C-terminal extensions involved in substrate recognition, localization, and protein interactions.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.