Thermo Fisher Scientific CD45 Monoclonal Antibody (1.11.32), PE
Bovine, Goat, Sheep에 반응하는 CD45 단일클론 항체로, PE 형광으로 표지되어 FACS 분석에 적합합니다. Protein A로 정제된 동결건조 형태이며, 4°C 암소 보관이 권장됩니다. 면역세포 표지 및 항원 신호전달 연구에 유용합니다.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: Neat
- Publications: View 6 publications
Miscellaneous PubMed (Misc)
- Tested Dilution: -
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Goat, Sheep |
| Published Species | Bovine, Human, Pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1.11.32 |
| Immunogen | Ovine efferent lymphatic duct lymphocytes |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 1% BSA, 5% sucrose |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_928391 |
Additional Formats
Product Specific Information
- Reconstitute with 1 mL of distilled water.
- After reconstitution, store undiluted at 4°C in the dark.
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
- Mouse anti-Sheep CD45 antibody (clone 1.11.32) recognizes ovine CD45 (Leukocyte Common Antigen), expressed on all ovine lymphocytes, macrophages, and granulocytes.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, comprising about 10% of lymphocyte surface proteins.
It is absent on non-hematopoietic cell lines and tissues.
CD45 plays a crucial role in lymphocyte development and antigen signaling by regulating Src-family kinases.
Multiple isoforms arise from alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions.
It regulates T- and B-cell receptor signaling and JAK kinase activity, influencing cytokine signaling, apoptosis, and integrin-mediated signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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