Thermo Fisher Scientific Hemoglobin Chimeric Recombinant Rabbit Monoclonal Antibody (2A1)

Applications

Product Specifications

Product Specific Information

Specificity:
Clone 2A1 exhibited polyreactive recognition of peptide 100–119 from the alpha subunit and peptide 100–119 from the beta subunit. It bound both Hb and the Hb-haptoglobin complex with equal efficiency, suggesting distinct binding sites on Hb for haptoglobin and 2A1. Additionally, 2A1 was able to distinguish between Hb and metHb.

Preparation:
Murine hybridomas were generated using spleen cells from aging (8–10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution, and antibody isotypes were determined using commercial reagents. Eight-week-old NZB/W F1 mice were immunized subcutaneously with 50 µg mHb antigen emulsified in Complete Freund’s Adjuvant.

Target Information

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci:
5′- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3′.

The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical, differing slightly in the 5′ untranslated regions and introns, but significantly in the 3′ untranslated regions. Two alpha chains plus two beta chains form HbA, which constitutes about 97% of normal adult hemoglobin. Alpha chains combined with delta chains form HbA2, which, along with fetal hemoglobin (HbF), makes up the remaining 3%. Alpha thalassemias result from deletions of one or both alpha genes (HBA2, HBA1) or from nondeletion mutations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.