Thermo Fisher Scientific CD45RB Monoclonal Antibody (PD7/26), Alexa Fluor 488, eBioscience
CD45RB 단백질을 인식하는 PD7/26 클론의 마우스 모노클론 항체로, Alexa Fluor 488 형광 표지. 인간 시료에 반응하며, IHC, ICC/IF, Flow Cytometry에 적용 가능. 0.5 mg/mL 농도의 액상 형태로 제공되며, 4°C 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 2 publications |
| Flow Cytometry (Flow) | Assay-dependent | 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PD7/26 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574449 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- Alexa Fluor Plus 555
- eFluor 660
- Custom conjugation available
Product Specific Information
Description:
The monoclonal antibody PD7/26 recognizes CD45RB, an isoform of the Leukocyte Common Antigen (LCA, CD45). CD45RB is a protein tyrosine phosphatase receptor expressed on most hematopoietic cells and absent on non-hematopoietic cells. Its expression is restricted to T cell subsets, B cells, NK cells, and myeloid cells. Regulatory T cells (Tregs) express low levels of CD45RB, correlating with increased migration to infection sites. High CD45RB expression induces IL-2 and IFN-γ production instead of IL-4 in vitro.
Applications Reported:
Reported for use in flow cytometry, immunohistochemistry (formalin-fixed paraffin-embedded tissue), microscopy, and immunocytochemistry.
Applications Tested:
Tested by IHC using low pH antigen retrieval at ≤10 µg/mL. Optimal dilution should be determined experimentally.
Excitation/Emission: 488 nm / 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45RB is a receptor-type protein tyrosine phosphatase and isoform of CD45 glycoprotein. CD45 plays a key role in lymphocyte development and antigen signaling by regulating Src-family kinases, promoting cell survival, and modulating integrin-mediated signal transduction. It is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in extracellular domains and expression patterns depending on cell type and physiological state. CD45RB is expressed in microglia and inflammatory cells and is essential in immunoreceptor signaling and apoptosis regulation. CD45 is expressed on all human hematopoietic cells except mature red blood cells.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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