Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (1B7), Alexa Fluor 488, eBioscience
인간 EpCAM(CD326)을 인식하는 Mouse Monoclonal Antibody로, Alexa Fluor 488 형광 표지된 제품입니다. Flow Cytometry 및 IHC 등 다양한 응용에 적합하며, 높은 특이성과 재현성을 제공합니다. 연구용 전용으로 4°C 암소 보관이 권장됩니다.
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Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (1B7), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 19 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1B7 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2815218 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- Biotin
- eFluor 660
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1) is a 40 kD cell-surface adhesion molecule involved in homophilic, calcium-independent cell-cell interactions. It is a type-I transmembrane protein expressed primarily on the basolateral surface of most epithelia.
Normal epithelia express low levels of EpCAM, whereas increased expression correlates with proliferation and epithelial-mesenchymal transition. EpCAM serves as a diagnostic marker for circulating metastatic carcinoma cells, while non-epithelial cancer cells do not express it.
Applications Reported: This 1B7 antibody is suitable for flow cytometric analysis.
Applications Tested: Pre-diluted and tested for flow cytometric analysis of A549 cells. Recommended usage is 5 µL (0.125 µg) per test (100 µL final volume). Cell count can range from 10⁵ to 10⁸ cells/test.
Excitation: 488 nm Emission: 519 nm Laser: Blue Laser
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial cells. It functions as a Ca²⁺-independent homotypic adhesion molecule and regulates cell cycle and proliferation through induction of c-myc and cyclin A/E genes.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated interactions, affecting epithelial differentiation and growth. Overexpression is linked to enhanced proliferation and carcinoma progression. It is highly expressed in human carcinomas and serves as a marker for epithelial-origin tumors.
Ep-CAM is found on the basolateral surface of most epithelia and distinguishes adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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