Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (1B7), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (1B7), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• Biotin
• eFluor 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1) is a 40 kD cell-surface adhesion molecule involved in homophilic, calcium-independent cell-cell interactions. It is a type-I transmembrane protein expressed primarily on the basolateral surface of most epithelia.
Normal epithelia express low levels of EpCAM, whereas increased expression correlates with proliferation and epithelial-mesenchymal transition. EpCAM serves as a diagnostic marker for circulating metastatic carcinoma cells, while non-epithelial cancer cells do not express it.

Applications Reported: This 1B7 antibody is suitable for flow cytometric analysis.
Applications Tested: Pre-diluted and tested for flow cytometric analysis of A549 cells. Recommended usage is 5 µL (0.125 µg) per test (100 µL final volume). Cell count can range from 10⁵ to 10⁸ cells/test.
Excitation: 488 nm Emission: 519 nm Laser: Blue Laser

Target Information

Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial cells. It functions as a Ca²⁺-independent homotypic adhesion molecule and regulates cell cycle and proliferation through induction of c-myc and cyclin A/E genes.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated interactions, affecting epithelial differentiation and growth. Overexpression is linked to enhanced proliferation and carcinoma progression. It is highly expressed in human carcinomas and serves as a marker for epithelial-origin tumors.
Ep-CAM is found on the basolateral surface of most epithelia and distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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