Thermo Fisher Scientific HSP70/HSC70 Monoclonal Antibody (N27F3-4), FITC
FITC로 표지된 HSP70/HSC70 단클론 항체로 다양한 종에 반응하며, WB, IHC, ICC, Flow Cytometry 등 다중 응용 가능. 단백질 G로 정제된 액상 형태로 4°C 암소 보관. 연구용으로 세포 스트레스 및 단백질 발현 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | |
| Immunocytochemistry (ICC/IF) | 1:50 | |
| Flow Cytometry (Flow) | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, C. elegans, Chicken, Fruit fly, Fish, Guinea pig, Hamster, Human, Mammal, Mouse, Non-human primate, Sheep, Plant, Pig, Rabbit, Rat, Xenopus |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N27F3-4 |
| Immunogen | Recombinant HSP70/HSC70 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.2, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931548 |
Additional Formats Available
- APC (Cat. No. MA5-45093)
- PerCP (Cat. No. MA5-45095)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45094 was sufficient for detection of HSP70/HSC70 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 72 kDa (HSP) and 73 kDa (HSC).
Target Information
HSPA4 (Heat Shock Protein Family A (Hsp70) Member 4) is a protein-coding gene associated with diseases such as Chagas Disease and Babesiosis. Related pathways include regulation of activated PAK-2p34 by proteasome-mediated degradation and cellular responses to stimuli.
HSPA8 (Heat Shock Protein Family A (Hsp70) Member 8) is also a protein-coding gene associated with auditory system disease and Borna disease. It participates in mRNA processing and selective autophagy. Gene Ontology annotations include RNA binding and ubiquitin protein ligase binding. An important paralog of this gene is HSPA2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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