Thermo Fisher Scientific IL-12 p40 Polyclonal Antibody
Goat polyclonal antibody against IL-12 p40 (IL12B) for WB, IHC, and ELISA applications. Reacts with mouse and rat, published in human. Supplied unconjugated in liquid form, 0.5 mg/mL concentration. Suitable for research use only.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.3–1 µg/mL | - |
| Immunohistochemistry (IHC) | – | View 1 publication |
| ELISA | – | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (EARQQNKYENYS) corresponding to internal amino acids of IL12B (aa 211–222) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10980247 |
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:16,000.
Target Information
IL12B (IL-12 p40) encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kDa cytokine receptor-like subunit encoded by this gene and a 35 kDa subunit encoded by IL12A.
This cytokine is expressed by activated macrophages and is essential for Th1 cell development. It supports memory/effector Th1 cells for long-term protection against intracellular pathogens.
Overexpression of IL12B has been observed in the central nervous system of multiple sclerosis (MS) patients, suggesting its role in disease pathogenesis. Promoter polymorphisms have been linked to the severity of atopic and non-atopic asthma in children.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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