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Norgen E.coli O157:H7 TaqMan PCR Detection Kits
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Norgen E.coli O157:H7 TaqMan PCR Detection Kits

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E.coli O157:H7를 정밀 검출하는 TaqMan PCR 키트로, 마스터믹스와 프라이머·프로브 세트 포함. 양성·음성 대조군 제공으로 실험 신뢰성 확보. -20°C 보관, 100회 반응용. 연구용 전용으로 식품, 수질 등 병원체 모니터링에 적합.

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Norgen · Norgen E.coli O157:H7 TaqMan PCR Detection Kits

Norgen E.coli O157:H7 TaqMan PCR Detection Kits

SKU: TM41350


Features and Benefits

  • Detection kits for E.coli O157:H7
  • Available in TaqMan format for analysis

E. coli O157:H7 is a rod-shaped, gram-negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis.
E. coli O157:H7 is recognized by its somatic (O157) and flagella (H7) antigens and is known to produce Shiga-like toxins causing severe symptoms.
Infection often results from consumption of undercooked meat, untreated water, or unpasteurized milk.


Product Options

E.coli O157:H7 TaqMan PCR Kit (100 reactions)

  • Ready-to-use format including Master Mix and PCR control
  • Specific Primer and Probe mix for the target pathogen
  • Positive and negative controls to confirm reagent integrity

E.coli O157:H7 TaqMan PCR Probe/Primer Set and Controls (100 reactions)

  • Specific Primer/Probe mix and Positive Control
  • Includes nuclease-free water
  • Compatible with Norgen’s PCR Master Mix (#28007) or user-supplied mix

For research use only and not intended for in vitro diagnostics.


Supporting Data

Figure 1. TaqMan PCR Positive Result (FAM + Cy5)

Both PCR signals (FAM and Cy5) above baseline indicate successful amplification.

OCR 추출 텍스트 요약:
상단 그래프: Amplification (FAM, Cy5), X축: Cycles, Y축: RFU
하단 그래프: Amplification (HEX), X축: Cycles, Y축: RFU

그래프 위치 제목 형광물질 X축 Y축
상단 Amplification FAM (파란색), Cy5 (빨간색) Cycles RFU
하단 Amplification HEX (초록색) Cycles RFU

Figure 2. TaqMan PCR Positive (Cy5) Result

PCR signals above baseline from Cy5 and HEX channels indicate successful PCR.

OCR 추출 텍스트 요약:
상단 그래프: Amplification (Cy5), X축: Cycles, Y축: RFU
하단 그래프: Amplification (HEX), X축: Cycles, Y축: RFU


Figure 3. TaqMan PCR Positive (FAM) Result

PCR signals above baseline from FAM and HEX channels indicate successful PCR.

OCR 추출 텍스트 요약:
상단 그래프: Amplification (FAM), X축: Cycles, Y축: RFU
하단 그래프: Amplification (HEX), X축: Cycles, Y축: RFU


Figure 4. TaqMan PCR Negative Result

No target DNA detected in FAM and Cy5 channels, but HEX amplification indicates successful PCR.

OCR 추출 텍스트 요약:
그래프 제목: Amplification
X축: Cycles (0–40), Y축: RFU (0–800)


Figure 5. TaqMan PCR Inhibition Result

No signal from FAM, Cy5, and HEX channels detected. Repeat sample preparation using recommended DNA purification kit.

OCR 추출 텍스트 요약:
상단 그래프: Amplification (FAM, Cy5), X축: Cycles, Y축: RFU
하단 그래프: Amplification (HEX), X축: Cycles, Y축: RFU


Storage Conditions and Product Stability

  • All kit components stable for 2 years after production
  • Store at -20°C upon arrival
  • Avoid repeated freeze-thaw cycles (>2×) of Master Mix and Positive Control
  • For intermittent use, aliquot reagents before freezing

Kit Components

Component Cat. TM41350 (100 rxns) Cat. TM41310 (100 rxns)
MDx TaqMan 2X PCR Master Mix 2 × 700 μL
E. coli O157:H7 Primer & Probe Mix 280 μL 280 μL
E. coli O157:H7 Positive Control 150 μL 150 μL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Product Insert 1 1

Documentation

Protocols

Safety Data Sheets



제품 이미지

  1. E.coli O157:H7 TaqMan PCR Kit Figure 1
  2. E.coli O157:H7 TaqMan PCR Kit Figure 2
  3. E.coli O157:H7 TaqMan PCR Kit Figure 3
  4. E.coli O157:H7 TaqMan PCR Kit Figure 4
  5. E.coli O157:H7 TaqMan PCR Kit Figure 5

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