Thermo Fisher Scientific alpha Synuclein Monoclonal Antibody (10H7), PerCP
Alpha Synuclein 단백질을 인식하는 PerCP 결합 단클론 항체로, Western blot, ICC/IF, ELISA, Dot blot에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며, 단백질 G로 정제된 액상 형태입니다. 파킨슨병 및 신경퇴행 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | 1:1,000 |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10H7 |
| Immunogen | Mouse alpha synuclein aggregate |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932296 |
Additional Formats
Product Specific Information
A 1:1,000 dilution of MA5-45842 was sufficient for detection of Alpha Synuclein in 15 µg of human brain cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Binds within the 101–130 aa region of human alpha synuclein, determined using Dot blot and ELISA with corresponding peptides.
Detects approximately 14 kDa band corresponding to Alpha Synuclein. Band at approximately 40 kDa is a trimer.
Does not detect truncated alpha Synuclein (aa 1–114).
Target Information
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking.
Alpha- and beta-synuclein selectively inhibit phospholipase D2, suggesting involvement in signaling pathways.
Defects in the SNCA gene, which encodes alpha-synuclein, are implicated in Parkinson’s disease pathogenesis.
Alpha-synuclein peptides are a major component of amyloid plaques in Alzheimer’s disease.
Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene.
Diseases associated with SNCA include Parkinson Disease 1, Autosomal Dominant, and Dementia, Lewy Body.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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