Thermo Fisher Scientific CDKN1A Monoclonal Antibody (OTI2D3), TrueMAB
CDKN1A(p21) 단백질을 인식하는 마우스 단일클론 항체로, Western blot에 최적화됨. 인간 시료 반응성, Lyophilized 형태로 제공. 재구성 및 결합 실험에 적합하며, p53 경로 연구에 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2D3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 2–164 of human CDKN1A produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Protein p21 is a tumor suppressor that inhibits Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53.
It regulates cell cycle progression by blocking transitions from G1 to S phase or G2 to mitosis after DNA damage, enabling DNA repair.
In the cytoplasm, p21 exhibits anti-apoptotic activity by inhibiting caspase 3 and kinases ASK1 and JNK.
P21 functions both as a tumor suppressor and oncogene depending on cellular context.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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