Thermo Fisher Scientific TSC1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:500

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:200

Product Specifications

Product Specific Information

• Positive Control: LO2 cell lysate, 293 cell lysate, A431 cell lysate, mouse pancreas tissue lysate, rat brain tissue lysate, LOVO, rat heart tissue, human fetal skeletal muscle tissue, human placenta tissue, HeLa cell lysates
• Subcellular Location: Cytoplasm, Membrane

Target Information

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), a serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors.
TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity.
Overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist, but its function is unknown.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

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